- 作 者：侯燕芝主编；张海燕，刘华副主编
- 出 版 社：北京：高等教育出版社
- 出版年份：2012
- ISBN：9787040334333
- 标注页数：108 页
- PDF页数：125 页
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Chapter 1 Introduction to the Medical Biology Laboratory 1
11 Objectives of a Teaching Medical Biology Laboratory 3
12 Laboratory Rules 3
13 Laboratory Safety 4
14 Common Laboratory Equipments 5
15 Experimental Methods in Medical Biology 5
16 Evaluation of Experimental Data and Laboratory Reports 18
Chapter 2 Experiments in Medical Biology 21
Experiment 1 The Use of Glass Pipets and Pipettors 23
Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins 27
Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay 29
Experiment 4 Quantitative Determination of Proteins by Folin-phenol Assay 32
Experiment 5 Determination of Proteins by UV Absorption 35
Experiment 6 Separation of Amino Acid Mixtures by Ion-exchange Chromatography 37
Experiment 7 Separation of Serumγ-globulins by Ion-exchange Chromatography 40
Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis 44
Experiment 9 Separation and Purity Determination of Serumγ-globulins by SDS-polyacrylamide Gel Electrophoresis 47
Experiment 10 Determination of Serum Glutamic-pyruvic Transaminase Activity 52
Experiment 11 Determination of Serum Urea Nitrogen Content 55
Experiment 12 Determination of Blood Glucose Concentration 58
Experiment 13 Subculture of Adhesion Cells 62
Experiment 14 Counting and Determination of Live/Dead Cells 64
Experiment 15 Preparation ofCell Samples for Observation of Cell Morphologies 67
Experiment 16 Mitosis and Meiosis of Cells 70
Experiment 17 Intracellular Distribution of Biomacromolecules 73
Experiment 18 Isolation of Subcellular Components 77
Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells 80
Experiment 20 Banding Pattern of Human Chromosomes 83
Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells 86
Experiment 22 Isolation,Amplification and Identification of the β-actin Gene Fragment from Mouse Liver Cells 91
Experiment 23 Purification of Target DNA and Generation of Recombinant DNA 97
Experiment 24 Transformation and Screening of Recombinant DNA 101
Experiment 25 Determination of Serum Proteins Using Western Blotting 106