- 作 者：Michael Lederer
- 出 版 社：
- 出版年份：1955
- ISBN：
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1．Introduction 1
Historical 1
Tiselius method 2
Scope of the Tiselius method 5
Terminology 7
References 8
2．Electrical Conduction of Solutions 10
Essentials of electrochemistry 10
Temperature variation of conductivity 11
Variation of conductivity with dilution 12
Independent mobility and absolute velocity of ions 14
3．Theoretical Considerations 17
Factors influencing the movement of particles in an electric field 17
Theory of paper electrophoresis 23
References 30
4．Technique of Paper Electrophoresis 31
Methods of supporting the filter paper 31
Other technical aspects 44
Preparative methods 46
References 49
5．Continuous Electrophoretic Separation 50
Principle 50
Apparatus 52
Some applications 57
References 60
6．Electrophoresis on the Cytoscale 61
7．Combination of Chromatography and Electrophoresis 63
Simultaneous chromatography and electrophoresis 63
Electrophoresis followed by chromatography 64
References 66
8．Organic Acids 67
Higher fatty acids 67
Keto acids 68
Di-and Tricarboxylic acids 68
References 69
9．Carbohydrates and Hydroxycompounds 70
Monosaccharides 71
Methyl ethers and disaccharides 73
Polysaccharides 75
Polyhydric alcohols and phenols 77
Flavonoids 79
References 80
10．Nitrogen Compounds 81
Amines 81
Aminosugars 81
Indole derivatives 81
Sulphanilamides 82
Amino acids and peptides 83
Two-dimensional separation of amino acids 86
DNP-amino acids 90
Guanido derivatives 89
Peptides 90
Iodothyronines and iodotyrosines 90
References 91
11．Alkaloids 92
12．Proteins 95
Details of the electrophoretic techniques 95
Quantitative evaluation of proteins on the paper 98
Detection of lipoids in serum fractions on the paper 106
Estimation of fibrinogen 108
Lysozyme 109
The subfractions of the serum globulins 109
Some clinical results 112
References 120
13．Nucleic Acids and Derivatives,and Phosphate Esters 125
Nucleic acids 125
Hydrolysis products of nucleic acids 126
Separation of ATP,ADP and related substances 130
Thiamine and thiamine phosphoric esters 132
Vitamin B2 and B6 groups 134
References 135
14．Antibiotics 136
15．Triterpenoids 138
16．Steroids 139
17．Dyes 141
18．Inorganic Compounds 143
Sequences of ions 143
Separations of analytical and preparative interest 147
References 155
19．Physico-chemical Studies 156
20．Electrophoresis in Packed Columns 162
Electrophoresis in a glass powder column 163
Electrophoresis in starch columns 164
Electrophoresis in a cellulose column 165
Electrophoresis in slabs of cellulose or starch 166
References 168
21．Electrophoresis Inside Gels 169
Electrophoresis in silica jelly 169
Electrophoresis in agar gel 174
References 186
22．Measurement of Diffusion Rates Inside Porous Media 187
23．Electrokinetic Ultrafiltration 189
Author Index 193
Subject Index 199