天然药物化学实验指导 英文版

Part Ⅰ Overview 3

Chapter One Introduction 3

Section One Contents and significance of natural medicinal chemistry research 3

Section Two The biosynthetic pathway of some important compounds 4

References 7

Chapter Two Extraction technology 9

Section One Solvent extraction 9

Section Two Distillation 25

Section Three Sublimation law 32

References 34

Chapter Three Separation technology 35

Section One Classical separation method 35

Section Two Modern separation method 78

Section Three Chromatographic separation method 82

References 97

Part Ⅱ Experiment 101

Experiment One The preparation and application of thin layer plate 101

Part One Objective and requirement 101

Part Two Preparation of thin layer plate 102

Part Three Application of thin layer chromatography 105

Part Four Examples 105

Part Five Questions 105

Experiment Two Extraction,purification and structure analysis of astragalus polysaccharides 106

Part One Experiment purpose 106

Part Two Experiment principle 106

Part Three Experimental instruments and reagents 106

Part Four Experimental content 107

References 117

Experimentation Three Extraction,separation and identification of esculin hydrate and esculetin in cortex fraxini 118

Part One Experimental principle 119

Part Two Experimental method 119

Part Three Thinking questions 120

Experimentation Four Extraction and separation of Anthraquinones from Rhubarb 121

Part One Purpose 121

Part Two Experimental principle 122

Part Three Experiment content 122

Part Four The experimental description and precautions 124

Part Five Thinking questions 124

References 125

Experimentation Five The Extraction and identification of rutin 126

Part One The progress of pharmacological research of rutin 126

Part Two The purpose of the experiment 128

Part Three Experimental principle 129

Part Four Experiment content 131

Part Five Experimental instructions and precautions 138

Part six Thinking questions 138

Part Seven Spectroscopic characterization of Quercetin 138

References 141

Experimentation Six The extraction of glycyrrhizic acid 142

Part One Experiment purpose 143

Part Two Experimental principle 143

Part Three Experiment content 144

Part Four Experimental instructions and precautions 145

Part Five Thinking question 146

References 146

Experiment Seven Extraction,isolation and formulation identification of Paeonol 147

Part One Purpose and requirements 148

Part Two Basic principles 148

Part Three Operations 149

Part Four Note 150

Part Five Thinking questions 151

References 151

Experimentation Eight Determination of Volatile Oil in Rhododendron fortunei 152

Part One Purpose 152

Part Two Experimental principle 152

Part Three Experimental content 153

Part Four Thinking questions 154

Referenees 154

Experimentation Nine Extraction and identification of Diosgenin 155

Part One Purpose and requirements 155

Part Two Prinpicle 155

Part Three Experimental content 156

Part Four Thinking questions 158

Experimentation Ten Extraction and separation of Berberine and Berbamine from three needles 159

Part One Purpose 160

Part Two Principle 160

Part Three Experiment content 161

Part Four The experimental description and precautions 163

Part Five Thinking questions 163

References 163

Experimentation Eleven Extraction of scopolamine and hyoscyamine from Yang Jinhua 164

Part One Purpose 164

Part Two Principle 164

Part Three Experiment content 166

Part Four The experimental description and precautions 168

Part Five Thinking questions 168

References 169

Experimentation Twelve Experimentation extraction of caffeine from tea 170

Part One Purpose 170

Part Two Principle 170

Part Three Experiment content 171

Part Four Thinking questions 173

Reference 173