- 作 者：Helen Kreuzer
- 出 版 社：ASM Press
- 出版年份：2008
- ISBN：1555814724
- 标注页数：488 页
- PDF页数：500 页
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PARTⅠ Laying the Foundation 1
1 An Overview of Biotechnology 3
2 Cell Properties and Processes 45
3 Genes，Genetics，and Geneticists 73
4 An Overview of Molecular Biology 97
5 Recombinant DNA Technology 137
PARTⅡ Classroom Activities 169
A.DNA Structure and Function 171
6 Student Activity：Constructing a Paper DNA Model 172
7 Student Activity：DNA Replication 174
8 Student Activity：From Genes to Proteins 176
Student Activity：Making Antisense：Regulating Gene Expression with RNA 179
9 Student Activity：Sizes of the Escherichia coli and Human Genomes 182
10 Student Activity：Extraction of Bacterial DNA 185
B.Manipulation and Analysis of DNA 187
11 Student Activity：DNA Scissors 188
12 Student Activity：DNA Goes to the Races 190
13 Student Activity：Restriction Analysis of Lambda DNA 194
14 Student Activity：Recombinant Paper Plasmids 199
15 Student Activity：Restriction Analysis Challenge 202
16 Student Activity：Detection of Specific DNA Sequences：PartⅠ.Fishing for DNA 205
Student Activity：Detection of Specific DNA Sequences：PartⅡ.Combining Restriction and Hybridization Analysis 207
Student Activity：Detection of Specific DNA Sequences：PartⅢ.Southern Hybridization 209
17 Student Activity：Paper PCR 210
18 Student Activity：DNA Sequencing：The Terminators 214
Reading：Chain Terminators as Antiviral Drugs 218
C.Transfer of Genetic Information 221
19 Student Activity：Transformation of Escherichia coli 222
20 Student Activity：Conjugative Transfer of Antibiotic Resistance in Escherichia coli 225
Reading：Gene Transfer and the Spread of Antibiotic Resistance 228
21 Student Activity：Transduction of an Antibiotic Resistance Gene 234
22 Student Activity：Agrobacterium tumefaciens：Nature’s Plant Genetic Engineer 238
D.Molecular Biology and Genetics 241
23 Student Activity：An Adventure in Dog Hair，Part Ⅰ 242
24 Student Activity：An Adventure in Dog Hair，Part Ⅱ：Yellow Labs 246
25 Student Activity：Human Molecular Genetics 250
Reading：Molecular Genetics of Cancer 258
26 Student Activity：Genetics in Action 264
E.Genomics 271
27 Student Activity：Comparing Genomes 272
Exercise 1：STRs Can Cause an RFLP 278
Exercise 2：PCR Can Reveal Differences at Microsatellite Loci 279
Reading：Mitochondrial DNA 281
28 Student Activity：Forensic DNA Typing 283
Exercise 1.A Mix-Up at the Hospital 285
Exercise 2.A Paternity Case 285
Exercise 3.The Case of the Bloody Knife 286
Reading：DNA Forensics：An Archaeological Case 289
29 Student Activity：Mapping a Disease Gene 290
Reading：The Human Genome Project：Its Science，Applications，and Issues 299
30 Student Activity：Microarray Analysis of Genome Expression 303
Reading：Personal Genomics 306
F.Bioinformatics and Evolutionary Analysis of Proteins 309
31 Student Activity：Testing for Amylase Activity 310
32 Student Activity：Electrophoresis of Amylase Samples 315
33 Student Activity：Constructing an Amylase Evolutionary Tree 318
34 Student Activity：Exploring Bioinformatics 322
PART Ⅲ Societal Issues 329
Introduction to Societal Issues 331
35 Student Activity：Facing the Forces：Science，Technology，and Society 332
Reading：The Nature of Science 338
36 Student Activity：Analyzing the Issues of Biotechnology：Cloning 341
Reading：Are Bioengineered Foods Safe？ 352
37 Student Activity：The Risks of Technology：Perception，Reality，or Both？ 356
Reading：Biotechnology Regulation in the United States 368
38 Student Activity：Making Decisions When There Is No Right Answer 372
39 Student Activity：Analyzing Ethical Dilemmas in Genetic Testing 377
Reading：Science，Law，and Politics 381
40 Student Activity：Analyzing Ethical Dilemmas in Gene Therapy 384
PART Ⅳ Appendixes 389
A Worksheets 390
B Laboratory Biosafety 453
C Aseptic Technique 455
Glossary 457
Index 469